Coding

Part:BBa_K3612010

Designed by: Jesus Alexandra Durand Calle   Group: iGEM20_UPCH_Peru   (2020-10-24)


GFPmut3b

GFP mut3b is a green fluorescent protein which will be used as a reporter for the characterization of our promoters Pasp, pMAV and pSHAB (BBa_K3612014, BBa_K3612013, BBa_K3612012) in the organism Pseudoalteromonas nigrifaciens.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The green fluorescent protein (GFP) is a protein of 238 amino acids (26.9 kDa) that exhibits fluorescence when exposed to light. It was first isolated from the jellyfish Aequorea victoria and is widely used as a reporter of the production of a broad range of proteins within biological systems. It can be mutated to regulate its stability or fluorescence. Our team will use GFP mut3b to characterize our three new promoters. (1)

References

Tsien RY (1998). "The green fluorescent protein". Annual Review of Biochemistry. 67: 509–44


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